期刊
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
卷 76, 期 5, 页码 1140-1149出版社
OXFORD UNIV PRESS
DOI: 10.1093/jac/dkab028
关键词
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资金
- Swiss Federal Food Safety and Veterinary Office (FSVO grant) [1.18.10]
- Swiss National Science Foundation (SNSF within the National Research Programme NRP72 'Antimicrobial Resistance') [177378]
- SNSF under the Joint Programming Initiative on Antimicrobial Resistance (JPIAMR) project [174273, JPIAMR/0002/2016]
- Fundação para a Ciência e a Tecnologia [JPIAMR/0002/2016] Funding Source: FCT
This study used whole-genome sequencing to analyze CPKp strains causing infections in hospitalized pets, finding them closely related to environmental strains. These findings confirmed nosocomial spread and highlighted veterinary hospitals as a potential source of CPKp, with potential spread to animals, the environment, and humans.
Objectives: Infections with carbapenem-resistant Enterobacterales (CRE) are an emerging problem in pets and a major threat to public health. We determined the genetic relationships among carbapenemase-producing Klebsiella pneumoniae (CPKp) strains causing infections in hospitalized pets in a veterinary clinic and those found in the environment. Methods: WGS was performed with both the Illumina and Nanopore platforms. Searches of genetic features were performed using several databases and bioinformatics tools, and phylogeny was assessed by whole-genome MLST (wgMLST) using SeqSphere and SNP calling with Snippy. Results: WGS analysis of the CPKp strains identified all environmental and almost all animal strains as the high-risk clone ST11, with the exception of two strains that belonged to ST307. All CPKp belonged to novel complex types (CTs) and carried a conjugative 63 kb IncL plasmid encoding the carbapenemase gene bla(OXA-48), yersiniabactin and other virulence factors. Although all CPKp ST11 strains carried additional similar IncR plasmids harbouring multiple antimicrobial resistance genes (ARGs), such as the plasmid-mediated bla(DHA-1) AmpC gene, some structural variations were observed. The two ST307 strains carried identical 156 kb MDR IncFIB(K) plasmids with several ARGs, including the bla(CTX-M-15) ESBL gene. Both wgMLST and cgSNP analysis confirmed that CPKp strains of the same ST were genetically highly related independent of the source of isolation. Conclusions: This study demonstrated that the clinical CPKp strains were highly related to those contaminating the clinical environment. These findings confirmed nosocomial spread and highlight veterinary hospitals as a source of CPKp, which may further spread to animals, the environment and humans.
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