期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 9, 页码 2773-2783出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c07798
关键词
walnut protein hydrolysate; scopolamine; cholinergic system; oxidative stress; peptidomics
资金
- National Key Research and Development Program of China [2017YFD0400201]
- Guangdong Provincial Key RD Programme [2020B020226005]
- Specific Fund Program for Basic and Applied Basic Research of Guangdong Province [2019A1515011952]
- Special Support Project of Guangxi Province for Innovation driven Development [AA17204075]
The study found that walnut protein hydrolysates (WPH) could improve memory deficits in mice by regulating the cholinergic system and oxidative stress, without obvious anti-inflammatory effects. By using peptidomics and in silico docking prediction, potential neuroprotective peptides were identified, such as FY and SGFDAE, which showed promising AchE and Keap1 inhibitory effects and neuroprotective capacity in scopolamine-induced zebrafish models.
The purpose of this study was to determine the neuroprotective effect and mechanism of walnut protein hydrolysates (WPH) against memory deficits induced by scopolamine in mice and further to validate the potent neuroprotective peptides identified by integrated approaches of in silico analysis and peptidomics in scopolamine-induced zebrafish. Results showed that a remarkable amelioration on behavioral performance was observed for oral administration of WPH, and disorders of cholinergic system and oxidative stress were normalized in the brains of mice also. Unfortunately, no obvious inflammatory response and anti-inflammatory effect were observed. Additionally, WPH significantly upregulated the expressions of antioxidant defense-related protein (Nrf2) and neurotrophic-related protein (BDNF and CREB). Furthermore, 20 peptides with relatively higher abundance and PeptideRanker scores were predicted by docking to AchE and Keap1. Among them, FY and SGFDAE with the highest binding affinities, -9.8 and -8.0 kcal/mol, were considered as the promising AchE and Keap1 inhibitors, respectively. They were further validated to have neuroprotective capacity in scopolamine-induced zebrafish, indicating that peptidomics and in silico prediction might be the effective approaches to screen neuroprotective peptides.
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