期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 9, 页码 2668-2678出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c07028
关键词
nanopesticide; vascular wilts; panama disease; pH-responsive release; carrier-mediated transport; amino acid transporter
资金
- Key Research and Development Project of Guangdong Province [2019B020217003]
- National Natural Science Foundation of China [31801777]
- Guangdong Provincial Innovation Team for General Key Technologies in Modern Agricultural Industry [2019KJ140]
- China Postdoctoral Science Foundation [2018M643104]
This study demonstrated the potential of transporter-mediated nanocarriers for targeted delivery of fungicides to control Fusarium wilt disease in bananas, resulting in a significant reduction in disease severity.
Fusarium wilt disease poses a serious threat to the global production of bananas. The targeted delivery of fungicides to banana phloem tissues may offer new hope for controlling this hard-to-treat vascular disease. In this study, fludioxonil (FLU)-loaded glycine methyl ester-conjugated polysuccinimide nanoparticles (PGA) were prepared with a loading efficiency (LE) of 27.9%. The obtained nanoparticles (FLU@PGA) exhibited pH-sensitive controlled release, specifically under an alkaline pH in plant phloem. In vivo experiments in potted bananas demonstrated that FLU@PGA can achieve the downward delivery of FLU to banana rhizomes and roots after foliar application, reducing disease severity by 50.4%. The phloem transport studies showed that the phloem loading of FLU@PGA was involved in an active transport mechanism at the organ level (castor bean seedlings). The observation of fluorescein-5-isothiocyanate cadaverine-labeled PGA nanocarriers showed that they could be absorbed by mesophyll cells and loaded into vascular tissues through the symplastic pathway. Furthermore, the interaction of FLU@PGA with the plant amino acid transporter AtLHT1 was observed to enhance transmembrane uptake at the cellular level (Xenopus oocytes). These results suggested that the phloem-targeted delivery of fungicide by transporter-mediated nanocarriers could be a promising new strategy for the management of Fusarium wilt in bananas.
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