期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 69, 期 7, 页码 2325-2336出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.0c06743
关键词
UHPLC; Q Exactive HF-X; thermally processed foods; hazardous compound
资金
- National Key Research and Development Program of China [2016YFD0400404]
- National Natural Science Foundation of China [32072247]
A rapid and reliable method based on UHPLC-QE/MS was established for the simultaneous quantification and validation of hazardous compounds in thermally processed foods. The method demonstrated good quantification recovery and excellent quantitative ability, with limits of quantification within a range from 0.1 to 50 ng/mL. The simultaneous determination of 16 hazardous compounds in different thermally processed foods was successfully achieved.
In this study, a rapid and reliable method based on ultrahigh-performance liquid chromatography coupled with Q Exactive HF-X mass spectrometry (UHPLC-QE/MS) was established for the simultaneous quantification and validation of acrylamide, 5-hydroxymethylfurfural, and 14 heterocyclic aromatic amines in thermally processed foods. With the optimization of the pretreatment method, all 16 hazardous compounds with different polarities were simultaneously extracted and purified by one-step purification. By studying various acquisition modes in detail, full MS + PRM detection using an electrospray ionization source in the positive mode gives an excellent-shaped chromatographic peak and thereby achieves a better quantitative ability for analytes in the matrix. This method demonstrated good quantification recovery in the range of 68.85-146.42%. The limits of quantification were within the range from 0.1 to 50 ng/mL. With the method proposed, the simultaneous determination of 16 hazardous compounds in different thermally processed foods was successfully applied. The all-fragment-ion approaches at high resolution have the ability to reduce false-positive peak detections arising from peak alignment software in the detection of samples significantly. The proposed isotope dilution UHPLC-QE/MS method was validated and demonstrated to be sensitive, accurate, and precise for the simultaneous quantification of multiple contaminants in one injection.
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