期刊
ISME JOURNAL
卷 15, 期 7, 页码 2012-2027出版社
SPRINGERNATURE
DOI: 10.1038/s41396-021-00901-5
关键词
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资金
- NSF through the University of Wisconsin Materials Research Science and Engineering Center [DMR-1720415]
- National Institutes of Health (NIGMS) [R35 GM137828]
- National Institutes of Health (NIAID) [R01 AI145939]
- UW-Madison Department of Medicine William A Craig Research Award
- Burroughs Wellcome Fund [1012299]
- Doris Duke Charitable Foundation [2017074]
The study explored the overall biofilm community structure in a three-membered community derived from a chronic wound, showing that priority effects and inter-bacterial competition significantly shape the community structure. The attachment of Citrobacter freundii to Candida albicans is mediated by mannose-binding lectins, and co-cultures of these two species trigger neutrophil death and inflammation. These results enhance understanding of fungal-bacterial interactions and their impact on wound healing.
Polymicrobial biofilms are a hallmark of chronic wound infection. The forces governing assembly and maturation of these microbial ecosystems are largely unexplored but the consequences on host response and clinical outcome can be significant. In the context of wound healing, formation of a biofilm and a stable microbial community structure is associated with impaired tissue repair resulting in a non-healing chronic wound. These types of wounds can persist for years simmering below the threshold of classically defined clinical infection (which includes heat, pain, redness, and swelling) and cycling through phases of recurrent infection. In the most severe outcome, amputation of lower extremities may occur if spreading infection ensues. Here we take an ecological perspective to study priority effects and competitive exclusion on overall biofilm community structure in a three-membered community comprised of strains of Staphylococcus aureus, Citrobacter freundii, and Candida albicans derived from a chronic wound. We show that both priority effects and inter-bacterial competition for binding to C. albicans biofilms significantly shape community structure on both abiotic and biotic substrates, such as ex vivo human skin wounds. We further show attachment of C. freundii to C. albicans is mediated by mannose-binding lectins. Co-cultures of C. freundii and C. albicans trigger the yeast-to-hyphae transition, resulting in a significant increase in neutrophil death and inflammation compared to either species alone. Collectively, the results presented here facilitate our understanding of fungal-bacterial interactions and their effects on host-microbe interactions, pathogenesis, and ultimately, wound healing.
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