Paenibacillus cymbidii sp. nov., isolated from Cymbidium goeringii roots

A Gram-stain-variable, aerobic, rod-shaped, endospore-forming strain R196(T)) was isolated from internal tissues of roots of Cymbidium goeringii. Cells were motile with peritrichous flagella. The colonies were light pink on tryptone soya agar medium. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R196(T) fell into a phylogenetic cluster belonging to the genus Paenibacillus. Strain R196(T) was closely related to Paenibacillus cavernae C4-5(T) and Paenibacillus contaminans CKOBP-6(T) with 93.6 and 93.3% sequence similarities, respectively. The major cellular polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids, two unidentified aminophospholipids and an unidentified aminolipid. The dominant respiratory quinone was MK-7. The main cellular fatty acids were anteiso-C-15 : 0 (53.01%), C-16 : 0 (13.04%) and iso-C-16 : 0 (10.80%). The genome size of R196T was 9.45 Mb, containing 7617 predicted protein-coding genes, with a DNA G+C content of 57.7 mol%. Based on the results of phenotypic, chemotaxonomic and whole-genome analyses, strain R196(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus cymbidii sp. nov. is proposed. The type strain is R196(T) (=ACCC 61713(T)=KCTC 33718(T)).

Automated summary

Strain R196(T) is a novel species of the genus Paenibacillus, named Paenibacillus cymbidii sp. nov., with distinctive cellular polar lipids and respiratory quinone composition. The genome of R196T contains 7617 predicted protein-coding genes, with a DNA G+C content of 57.7 mol%.