Nocardia bovistercoris sp. nov., an actinobacterium isolated from cow dung

A novel actinobacterium, designated strain NEAU-351(T), was isolated from cow dung collected from Shangzhi, Heilongjiang Province, northeast PR China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-351T belonged to the genus Nocardia, with the highest similarity (98.96 %) to Nocar-dia takedensis DSM 44801(T) and less than 98.0 % identity with other type strains of the genus Nocardia. The polar lipids con -sisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major menaquinone was observed to contain MK-8(H-4, omega-cycl) (78.2 %). The fatty acid profile mainly consisted of C-16.0, C-18.1 omega 9c and 10-methyl C-18.0. Mycolic acids were present. The genomic DNA G+C content of strain NEAU-351T was 68.1 mol%. In addition, the average nucleotide identity values between strain NEAU-351T and its reference strains, Nocardia takedensis DSM 44801T and Nocardia arizonensis NBRC 108935(T), were found to be 81.4 and 82.9 %, respectively, and the level of digital DNA-DNA hybridization between them were 24.8 % (22.5-27.3 %) and 26.3 % (24-28.8 %), respectively. Here we report on the taxonomic characterization and classification of the isolate and propose that strain NEAU-351(T) represents a new species of the genus Nocardia, for which the name Nocardia bovistercoris is proposed. The type strain is NEAU-351(T) (=CCTCC AA 2019090(T)=DSM 110681(T)).

Automated summary

The novel actinobacterium strain NEAU-351(T) isolated from cow dung in Heilongjiang Province, China belongs to the genus Nocardia and represents a new species named Nocardia bovistercoris. It exhibits specific characteristics including lipid composition, major menaquinone content, fatty acid profile, and genomic DNA G+C content.