Criibacterium bergeronii gen. nov., sp. nov., a new member of the family Peptostreptococcaceae, isolated from human clinical samples

A rod-shaped, motile anaerobic bacterium, designated CCRI-22567(T), was isolated from a vaginal sample of a woman diag-nosed with bacterial vaginosis and subjected to a polyphasic taxonomic study. The novel strain was capable of growth at 30-42 degrees C (optimum, 42 degrees C), at pH 5.5-8.5 (optimum, pH 7.0-7.5) and in the presence of 0-1.5 % (w/v) NaCl (optimally at 0.5 % NaCl). The phylogenetic trees based on 16S rRNA gene sequences showed that strain CCRI-22567(T) forms a distinct evolu-tionary lineage independent of other taxa in the family Peptostreptococcaceae. Strain CCRI-22567T exhibited 90.1 % 16S rRNA gene sequence similarity to Peptoanaerobacter stomatis ACC19aT and 89.7 % to Eubacterium yurii subsp. schtitka ATCC 43716. The three closest organisms with an available whole genome were compared to strain CCRI-22567(T) for genomic relatedness assessment. The genomic average nucleotide identities (OrthoANIu) obtained with Peptoanaerobacter stomatis ACC19a(T), Eubac-terium yurii subsp. margaretiae ATCC 43715 and Filifactor alocis ATCC 35896T were 71.8, 70.3 and 69.6 %, respectively. Strain CCRI-22567(T) contained C18.1 omega 9c and C18.1 omega 9c DMA as the major fatty acids. The DNA G+C content of strain CCRI-22567T based on its genome sequence was 33.8 mol%. On the basis of the phylogenetic, chemotaxonomic and other phenotypic properties, strain CCRI-22567(T) is considered to represent a new genus and species within the family Peptostreptococcaceae, for which the name Criibacterium bergeronii gen. nov., sp. nov., is proposed. The type strain of Criibacterium bergeronii is CCRI-22567(T) (=LMG 31278(T)=DSM 107614(T)=CCUG 72594(T)).

Automated summary

A rod-shaped, motile anaerobic bacterium, designated CCRI-22567(T), was isolated from a vaginal sample of a woman diagnosed with bacterial vaginosis. Based on phylogenetic analysis, chemotaxonomy, and other phenotypic properties, strain CCRI-22567(T) represents a new genus and species within the family Peptostreptococcaceae, named Criibacterium bergeronii gen. nov., sp. nov.