期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 594, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.ijpharm.2020.120167
关键词
Oral vaccination; Microfold cells; Particulate nanocarriers; Optical tissue clearing; Quantitative analyses
资金
- Australian Research Council [LP150100032]
- Australian Research Council Center of Excellence in Convergent Bio-Nano Science and Technology
- Australian Research Council [LP150100032] Funding Source: Australian Research Council
Various nanoparticulate systems have been engineered to enhance the bioavailability of orally administrated vaccines by targeting microfold cells (M cells) within Peyer's patches (PPs). A novel clearing-based imaging technique was developed to quantitatively determine the distribution of nanoparticles within ex vivo murine PPs, revealing enhanced translocation of M cell-targeted nanoparticles compared to non-targeted ones. This approach may aid in the design of improved oral vaccines.
Diverse nanoparticulate systems have been engineered as vehicles towards enhancing the bioavailability of orally administrated vaccines. Substantial evidence suggests that targeting microfold cells (M cells) within Peyer's patches (PPs) is a prerequisite for vaccine-loaded nanocarriers to induce an effective antigen-specific immune response. Improved understanding of the contribution of M cells to sampling luminal nanoparticles into the underlying gut associated lymphoid tissues would accelerate the development of oral vaccine formulations. Herein, a novel clearing-based whole tissue mount/imaging technique was developed to enable the specific distribution of nanoparticles within ex vivo murine PPs to be quantitatively determined at the cellular level. This revealed that 200 nm nanoparticles modified with M cell targeting ligands (lectin Ulex europaeus agglutinin-1, UEA-1) were translocated into subepithelial domes 7.6 and 16.3 times greater than the non-targeted ones at 60 min and 120 min, respectively. This approach provides a new methodology to quantitatively investigate the transcytotic activity of M cells for particulate formulations, which may aid in the design of improved oral vaccines.
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