4.7 Article

The Role of the Pathogen Dose and PI3Kγ in Immunometabolic Reprogramming of Microglia for Innate Immune Memory

期刊

出版社

MDPI
DOI: 10.3390/ijms22052578

关键词

microglia; immunometabolism; innate immune memory; LPS; PI3Kγ glycolysis; pentose phosphate pathway; OXPHOS; OCR; ECAR

资金

  1. Deutsche Forschungsgemeinschaft (DFG) [RTG 1715, RTG 2155]
  2. Else-Kroner-Forschungskolleg AntiAge
  3. PRIMAL consortium - Federal Ministry for Education and Research (Bundesministerium fur Bildung und Forschung, BMBF) [01GL1746E]

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Microglia exhibit long-term response changes indicative of innate immune memory, with patterns of trained immunity and immune tolerance after low and high dose challenges with pathogen-associated molecular patterns, respectively. Immunometabolic reprogramming is involved in IIM in a dose-dependent manner via the glycolytic pathway, oxygen consumption and ATP production: ULP increases it, while HP reduces it.
Microglia, the innate immune cells of the CNS, exhibit long-term response changes indicative of innate immune memory (IIM). Our previous studies revealed IIM patterns of microglia with opposing immune phenotypes: trained immunity after a low dose and immune tolerance after a high dose challenge with pathogen-associated molecular patterns (PAMP). Compelling evidence shows that innate immune cells adopt features of IIM via immunometabolic control. However, immunometabolic reprogramming involved in the regulation of IIM in microglia has not been fully addressed. Here, we evaluated the impact of dose-dependent microglial priming with ultra-low (ULP, 1 fg/mL) and high (HP, 100 ng/mL) lipopolysaccharide (LPS) doses on immunometabolic rewiring. Furthermore, we addressed the role of PI3K gamma on immunometabolic control using naive primary microglia derived from newborn wild-type mice, PI3K gamma-deficient mice and mice carrying a targeted mutation causing loss of lipid kinase activity. We found that ULP-induced IIM triggered an enhancement of oxygen consumption and ATP production. In contrast, HP was followed by suppressed oxygen consumption and glycolytic activity indicative of immune tolerance. PI3K gamma inhibited glycolysis due to modulation of cAMP-dependent pathways. However, no impact of specific PI3K gamma signaling on immunometabolic rewiring due to dose-dependent LPS priming was detected. In conclusion, immunometabolic reprogramming of microglia is involved in IIM in a dose-dependent manner via the glycolytic pathway, oxygen consumption and ATP production: ULP (ultra-low-dose priming) increases it, while HP reduces it.

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