期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/ijms22041772
关键词
middle east respiratory syndrome coronavirus (MERS-CoV); severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); molecular diagnostics; reverse transcription loop-mediated isothermal amplification (RT-LAMP)
资金
- LG Chem's Early Career Faculty Research Program
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2018R1C1B3007409]
- Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) from the Ministry of Trade, Industry and Energy [20194030202330]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20194030202330] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2018R1C1B3007409, 4120200413631] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
The study introduces a new nucleic acid detection method that allows fast, sensitive, specific, and colorimetric detection of coronavirus. This method combines reverse transcription loop-mediated amplification with a toehold reaction, enhancing the sensitivity and speed of RNA virus detection.
A rapid, sensitive and simple point-of-care (POC) nucleic acid diagnostic test is needed to prevent spread of infectious diseases. Paper-based toehold reaction, a recently emerged colorimetric POC nucleic acid diagnostic test, has been widely used for pathogen detection and microbiome profiling. Here, we introduce an amplification method called reverse transcription loop-mediated amplification (RT-LAMP) prior to the toehold reaction and modify it to enable more sensitive and faster colorimetric detection of RNA viruses. We show that incorporating the modified RT-LAMP to the toehold reaction detects as few as 120 copies of coronavirus RNA in 70 min. Cross-reactivity test against other coronaviruses indicates this toehold reaction with the modified RT-LAMP is highly specific to the target RNA. Overall, the paper-based toehold switch sensors with the modified RT-LAMP allow fast, sensitive, specific and colorimetric coronavirus detection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据