期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/ijms22041697
关键词
bacterial cell division; Staphylococcus aureus; FtsZ; high-speed atomic force microscopy
资金
- Japan Society for the Promotion of Science [15J00589, 25440022, 25121719, 26102526, 16H00783, 17H05732, 18K06094, 18H01837, 19H05389, 19KK0180]
- Grants-in-Aid for Scientific Research [15J00589, 18K06094, 17H05732, 16H00783, 25440022, 19KK0180, 18H01837, 26102526] Funding Source: KAKEN
FtsZ is a key protein in bacterial cell division, and high-speed AFM allows visualization of its filament formation and dissociation process. Analysis shows that FtsZt filaments have different growth and dissociation rates under different conditions, and prefer straight shapes compared to FtsZf filaments.
FtsZ is a key protein in bacterial cell division and is assembled into filamentous architectures. FtsZ filaments are thought to regulate bacterial cell division and have been investigated using many types of imaging techniques such as atomic force microscopy (AFM), but the time scale of the method was too long to trace the filament formation process. Development of high-speed AFM enables us to achieve sub-second time resolution and visualize the formation and dissociation process of FtsZ filaments. The analysis of the growth and dissociation rates of the C-terminal truncated FtsZ (FtsZt) filaments indicate the net growth and dissociation of FtsZt filaments in the growth and dissociation conditions, respectively. We also analyzed the curvatures of the full-length FtsZ (FtsZf) and FtsZt filaments, and the comparative analysis indicated the straight-shape preference of the FtsZt filaments than those of FtsZf. These findings provide insights into the fundamental dynamic behavior of FtsZ protofilaments and bacterial cell division.
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