期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 22, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/ijms22042198
关键词
keloid scar; hypertrophic scar; dermal fibroblast; epidermal growth factor; ECM remodeling; traction force
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2017R1D1AB03029770, 2016K1A4A3914725]
- Brain Pool Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2019H1D3A2A02102074]
- Daewoong Pharmaceutical Company
- National Research Foundation of Korea [2019H1D3A2A02102074] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
This study revealed the molecular characteristics and high fibrotic activity of keloid dermal fibroblasts at cell-matrix interfaces. Additionally, downregulation of anabolic collagen-cross-linking enzymes through epidermal growth factor led to decreased fibrotic phenotypes and cell motility.
Keloid and hypertrophic scars are skin fibrosis-associated disorders that exhibit an uncontrollable proliferation of fibroblasts and their subsequent contribution to the excessive accumulation of extracellular matrix (ECM) in the dermis. In this study, to elucidate the underlying mechanisms, we investigated the pivotal roles of epidermal growth factor (EGF) in modulating fibrotic phenotypes of keloid and hypertrophic dermal fibroblasts. Our initial findings revealed the molecular signatures of keloid dermal fibroblasts and showed the highest degree of skin fibrosis markers, ECM remodeling, anabolic collagen-cross-linking enzymes, such as lysyl oxidase (LOX) and four LOX-like family enzymes, migration ability, and cell-matrix traction force, at cell-matrix interfaces. Furthermore, we observed significant EGF-mediated downregulation of anabolic collagen-cross-linking enzymes, resulting in amelioration of fibrotic phenotypes and a decrease in cell motility measured according to the cell-matrix traction force. These findings offer insight into the important roles of EGF-mediated cell-matrix interactions at the cell-matrix interface, as well as ECM remodeling. Furthermore, the results suggest their contribution to the reduction of fibrotic phenotypes in keloid dermal fibroblasts, which could lead to the development of therapeutic modalities to prevent or reduce scar tissue formation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据