期刊
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
卷 135, 期 4, 页码 1213-1224出版社
SPRINGER
DOI: 10.1007/s00414-021-02525-y
关键词
Forensic medicine; 16S rDNA sequencing; Microbiome-based methods; Vaginal fluids; Bio-geographical factors
资金
- Natural Science Foundation of Guangdong Province [2020A1515010938]
- Science and Technology Program of Guangzhou, China [2019030016]
- Opening Fund of Shanghai Key Laboratory of Forensic Medicine (Institute of Forensic Science, Ministry of Justice, China) [KF1914]
Utilizing high-throughput sequencing technology, this study analyzed the microbial composition of vaginal samples from different regions, revealing potential influences of biogeographical factors on the relative abundance of vaginal microflora. While the dominant bacteria composition remained similar, a random forest algorithm showed promise in distinguishing vaginal fluids from populations of different regions. Microbiome-based methods may provide more personalized information for tracing the origin of body fluids.
The human microbiome is expected to be a new and promising tool for classification of human epithelial materials. Vaginal fluids are one of the most common biological samples in forensic sexual assault cases, and its identification is crucial to accurately determine the nature of the case. With the development of molecular biology technologies, the concept of vaginal microflora in different physiological states, ethnic groups, and geography is constantly improved. In this study, we conducted high-throughput sequencing of the V3-V4 hypervariable regions of the 16S rRNA gene in vaginal samples from Henan, Guangdong, and Xinjiang populations, in an attempt to reveal more information about the vaginal microflora in different regions. The results showed that the bio-geographical factors might affect the relative abundance of some vaginal microflora, but there was no significant difference in the composition of dominant bacteria in the vagina, which was mainly composed of Lactobacillus and Gardnerella. However, prediction models based on the random forest algorithm suggested that we might be able to distinguish vaginal fluids from populations of different regions according to the species-level OTUs in low abundance. It is promising that microbiome-based methods could provide more personal information when being attempted to trace the origin of body fluids.
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