期刊
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
卷 57, 期 1, 页码 541-551出版社
WILEY
DOI: 10.1111/ijfs.15026
关键词
Anti-hyperglycaemic effects; burdock leaf flavonoids; morin; alpha-amylase; alpha-glucosidase
资金
- Jiangsu Subei Science and Technology Project [XZ-SZ201825]
Burdock leaf flavonoids (BLF) showed inhibitory effects on α-amylase (α-A) and α-glucosidase (α-G) activities, with morin identified as the key active compound. Addition of BLF and morin significantly reduced rapidly digestible starch content in wheat flour. This suggests that BLF can be a new functional ingredient for designing lower glycemic-response foods.
Burdock (Arctium lappa L.) leaves are a polyphenol-rich and underutilised by-product of burdock. To exploit the use of burdock leaves, inhibitory effects of burdock leaf flavonoids (BLF) on alpha-amylase (alpha-A) and alpha-glucosidase (alpha-G) activities and starch digestion were investigated. The findings revealed that BLF exhibited promising alpha-A (IC50 value of 92.01 mu g mL(-1)) and alpha-G (IC50 value of 29.49 mu g mL(-1)) inhibitions in a mixed-type manner. Fourteen compounds in BLF were identified, and apigenin glycosides, morin, kaempferol and quercetin 3-O-rhamnoside were the predominant compounds in BLF. The key active compound existing in BLF was morin which possessed the highest inhibition of alpha-A and alpha-G activities followed by quercetin 3-O-rhamnoside and kaempferol. Kinetic and spectroscopic studies verified that morin could bind to the two carbohydrate digestive enzymes to form the complexes, causing declines in alpha-A and alpha-G activities. The results of starch digestion in vitro showed that the addition of 4% BLF and morin significantly reduced the content of rapidly digestible starch in wheat flour. The above results suggest that BLF can become a new functional ingredient which may help in the design of lower glycaemic-response foods.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据