期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
卷 32, 期 7, 页码 1500-1512出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09603123.2021.1893282
关键词
Dust; Dermatophagoides; Multiplex PCR
资金
- Foundation for Science and Technology (FCT) [SFRH//BD/136192/2018]
- European Regional Development Fund (ERDF), under the Portugal [UIDB/00709/2020]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/136192/2018] Funding Source: FCT
The study aimed to develop a fast and accessible multiplex PCR technique for detecting and distinguishing two relevant House Dust Mite species in house dust. The results showed high sensitivity of the technique in detecting both species, indicating its potential for practical application.
Dermatophagoides pteronyssinus and Dermatophagoides farinae are the most common House Dust Mite (HDM) species in home environments worldwide and responsible for HDM allergy. Since the prevalence of HDM-related clinical conditions is linked to exposure to the mite itself, the detection of HDM in the human households gains importance. We aimed to develop a fast and accessible multiplex PCR to detect and distinguish two relevant HDM species in house dust. New primers were designed, and sensitivity analysis was performed. Sequencing of PCR products was also performed to confirm the method's specificity. The limit of detection of the multiplex PCR for both species was as low as 30 pg mu L-1. The application of the multiplex PCR to dust samples also resulted in the identification of both species with high sensitivity. The protocol required small amount of template, reagents and a short reaction time thus presenting an alternative to classically used techniques for HDM identification.
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