期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
卷 32, 期 6, 页码 1382-1392出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09603123.2021.1885632
关键词
Quinolone resistance; food animals; plasmid-mediated quinolone resistance; Escherichia coli; Klebsiella pneumoniae
This study investigated quinolone resistance determinants in Klebsiella pneumoniae and Escherichia coli isolates from human and animal sources, showing a high prevalence of plasmid-mediated quinolone resistance (PMQR) genes. The presence of PMQR genes in food-producing animal isolates is of great concern, indicating a widespread issue of antibiotic resistance.
This study characterized quinolone (Q) resistance determinants in a series of Klebsiella pneumoniae (n = 26) and Escherichia coli (n = 19) isolates of human and animal origin. The presence of plasmid-mediated quinolone resistance (PMQR) and carabpenemase genes was examined by PCR. The quinolone resistance-determining regions (QRDRs) of gyrA and parC genes were sequenced. Thirty-three isolates had ciprofloxacin MIC >= 8 mg/l. About 34.6% and 10.5% of K. pneumoniae and E. coli isolates were ESBL producers respectively. The PMQR genes were detected in 77% (n = 35) of isolates. The oqxAB was the most prevalent PMQR gene being identified in all K. pneumoniae isolates, followed by aac(6MODIFIER LETTER PRIME)-Ib-cr (34.6%), qnrS (23%) and qnrB (7.7%). The most frequently detected gene among E. coli isolates was qnrS (36.8%) followed by aac(6 ')-Ib-cr (10.5%) and qepA (5.2%). All Q resistant isolates harbored amino acid substitutions in both GyrA and ParC QRDRs. High prevalence of PMQR genes among food-producing animal isolates is an issue of great concern.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据