Antioxidation, anti-inflammation and anti-fibrosis effect of phosphorylated polysaccharides from Pleurotus djamor mycelia on adenine-induced chronic renal failure mice

The mycelia polysaccharides (MPS) from Pleurotus djamor were prepared and purified by anion exchange column chromatography, and the phosphate content of phosphorylated MPS (PMPS) was 15.22 +/- 0.37%. FT-IR spectra, HPLC and H-1 and C-13-NMR results showed the PMPS contained alpha-pyranose structure and the peak area percentage composition of galacturonic acid and glucose were 13.01% and 85.82%, respectively. Animal experiment investigated the antioxidant, anti-inflammation, anti-fibrosis effects of PMPS on kidney in adenine-induced chronic renal failure (CRF) mice. All results including serum biochemical indices, histopathological observation, qRT-PCR, western blotting, immunohistochemical staining manifested the kidney injury could be remitted by PMPS interventions. This experiment suggested that PMPS could remit CRF and other kidney injury related diseases as one kind of dietary supplements and functional foods without toxic side effects. (C) 2020 Published by Elsevier B.V.

Automated summary

The study focused on the extraction and purification of PMPS from Pleurotus djamor, and investigated its antioxidant, anti-inflammatory, and anti-fibrosis effects in adenine-induced chronic renal failure (CRF) mice. Results showed that PMPS interventions could ameliorate kidney injury, suggesting its potential use as dietary supplements and functional foods for CRF and related kidney diseases without toxic side effects.