Isolation of an acidic polysaccharide from the flowers of Leucosceptrum canum Smith and its immunomodulatory activity evaluation

A water-soluble polysaccharide (LCP-05) was isolated from the flowers of Leucosceptrum canum Smith. LCP-05 was an acidic polysaccharide with a molecular weight of approximately 8.9 kDa. Monosaccharide composition analysis indicated that LCP-05 was composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara in a molar ratio of 0.83:1.68:0.33:2.15:1.00:1.45:1.22. The framework of LCP-05 was speculated to be a branched rhamnogalacturonan with the backbone consisting of alpha-1,2,4-linked Rhap and alpha-1,4-linked GalAp, and bearing branches at the O-4 position of the Rha residues. The side chains are terminated primarily with the Araf and Glcp residues. LCP-05 was found to be able to significantly induce the production of NO, IL-6, and TNF-alpha in RAW264.7 cells, and to induce RAW264.7 cell's suppressive effect on both cell growth and cell migration of 4T1 mammary breast cancer cells. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

A water-soluble acidic polysaccharide (LCP-05) was isolated from Leucosceptrum canum Smith with a molecular weight of approximately 8.9 kDa. LCP-05 is composed of Man, Rha, GlcA, GalA, Glc, Gal and Ara. It has been found to significantly induce the production of NO, IL-6, and TNF-alpha in RAW264.7 cells, as well as have a suppressive effect on cell growth and migration of mammary breast cancer cells.