Distinguishing normal and aggregated alpha-synuclein interaction on gold nanorod incorporated zinc oxide nanocomposite by electrochemical technique

Misfolding and accumulation of the protein alpha synuclein in the brain cells characterize Parkinson's disease (PD). Electrochemical based aluminum interdigitated electrodes (ALIDEs) was fabricated by using conventional photolithography method and modified the surfaces with zinc oxide and gold nanorod by using spin coating method for the analysis of PD protein biomarker. The device surface modified with gold nanorod of 25 nm diameter was used. The bare devices and the surface modified devices were characterized by Scanning Electron Microscope, 3D-Profilometer, Atomic Force Microscope and high-power microscope. The above measurement was also performed to measure the interaction of antibody with aggregated alpha-synuclein for normal, aggregated and aggregated alpha synuclein in human serum and distinguished against 3 control proteins (PARK1, DJ-1 and Factor IX). The detection limit for normal alpha synuclein was 1 f. with the sensitivity of 1 f. on a linear regression (R-2 = 0.9759). The detection limit for aggregated alpha synuclein was 10 aM with the sensitivity of 1 aM on a linear regression (R-2 = 0.9797). Also, the detection limit of aggregated alpha synuclein in serum was 10 aMwith the sensitivity of 1 aM on a linear regression (R-2 = 0.9739). These results however indicate that, serum has only minimal amount of alpha synuclein. (C) 2021 Elsevier B.V. All rights reserved.

Automated summary

In this study, electrochemical devices were used for the analysis of Parkinson's disease protein biomarkers, revealing detection limits of 1 f for normal alpha synuclein and 10 aM for aggregated alpha synuclein, with minimal amounts of aggregated alpha synuclein in serum.