期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 160, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2020.113124
关键词
Oil palm empty fruit bunch fiber; Xylooligosaccharide; Prebiotic; Lactobacillus plantarum; Bifidobacterium bifidum
资金
- Thai Government [WU61107]
- Walailak University [WU60603]
- Thailand Research Fund [RTA6080010]
Hemicellulose was successfully recovered from oil palm empty fruit bunch fiber and used to produce xylooligosaccharides prebiotics, with optimized conditions of sequential peracetic acid and alkaline peroxide treatments. The enriched hemicellulose yield was achieved through Taguchi experimental design.
Hemicellulose was recovered from oil palm empty fruit bunch (EFB) fiber and used for producing xylooligosaccharides (XOS) prebiotics. EFB fibers were treated sequentially with peracetic acid (PA) and alkaline peroxide (AP). The PA treatment removed similar to 50 % lignin. The subsequent AP treatment recovered the hemicellulose. The AP treatment was optimized using Taguchi experiment design with L16 orthogonal array consisting of 5 factors (NaOH concentration, H2O2 concentration, MgSO4 concentration, incubation temperature, treatment time) and 4 levels. Data from 16 experimental runs showed that the main effect of the factors on percentage hemicellulose recovery was in the order: NaOH > MgSO4 > incubation temperature > time > H2O2. The optimal conditions for hemicellulose isolation were: 15 % w/v NaOH in the reaction system (3 g NaOH/g delignified EFB), 1% w/v MgSO4 (0.2 g MgSO4/g delignified EFB), 45 degrees C incubation temperature and a 3 h reaction time. The PA-AP treatments solubilized nearly 53 % of the original dry mass of EFB. The enriched hemicellulose yield on EFB was 18.4 % by weight. Acid hydrolysis of the released hemicellulose produced xylose (44.1 %), glucose (17.6 %), arabinose (2.2 %), galactose (2.2 %), and mannose (4.4 %). Thermomyces lenuginosus xylanase (294.4 U L-1) was used to produce XOS from hemicellulose at 55 degrees C in a 12-h reaction. The XOS were assessed as substrates for two probiotic bacteria found in human gastrointestinal tract. The probiotic Lactobacillus plantarum WU-P19 utilized XOS better than Bifidobacterium bifidum TISTR 2129. Coculture of the two bacteria was shown to produce mixed fatty acids.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据