期刊
IMMUNITY
卷 54, 期 3, 页码 542-+出版社
CELL PRESS
DOI: 10.1016/j.immuni.2021.02.001
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类别
资金
- Emory University Integrated Cellular Imaging Microscopy Core
- National Institutes of Health [RO1 AI148378-01S1]
- Fast Grants award [2166]
- NCRR/NIH [P51 OD011132]
- NIH [P51 OD011132, S10 OD026799]
The study demonstrates that MVA/S vaccination induces strong neutralizing antibody and T cell responses, providing protection against SARS-CoV-2 in mice and macaques. Single-cell RNA sequencing analysis of lung cells reveals that the vaccination also protects macaques from infection-induced inflammation and B cell abnormalities.
A combination of vaccination approaches will likely be necessary to fully control the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Here, we show that modified vaccinia Ankara (MVA) vectors expressing membrane-anchored pre-fusion stabilized spike (MVA/S) but not secreted S1 induced strong neutralizing antibody responses against SARS-CoV-2 in mice. In macaques, the MVA/S vaccination induced strong neutralizing antibodies and CD8(+) T cell responses, and conferred protection from SARS-CoV-2 infection and virus replication in the lungs as early as day 2 following intranasal and intratracheal challenge. Single-cell RNA sequencing analysis of lung cells on day 4 after infection revealed that MVA/S vaccination also protected macaques from infection-induced inflammation and B cell abnormalities and lowered induction of interferon-stimulated genes. These results demonstrate that MVA/S vaccination induces neutralizing antibodies and CD8(+) T cells in the blood and lungs and is a potential vaccine candidate for SARS-CoV-2.
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