期刊
GUT
卷 71, 期 2, 页码 357-371出版社
BMJ PUBLISHING GROUP
DOI: 10.1136/gutjnl-2020-321952
关键词
pancreatic cancer; stem cells
资金
- National Natural Science Foundation of China [82030092, 81720108028, 82072657, 81802432, 82072716, 81802433, 82072659, 81871968, 81871978]
- Tianjin Prominent Talents
- Tianjin Eminent Scholars
- Tianjin Natural Science Fundation [20JCQNJC01330, 18JCJQJC47800, 19JCJQJC63100, 19JCYBJC26200]
- Tianjin Postgraduate Research and Innovation Project [2019YJSB104]
- Tianjin Research Innovation Project for Postgraduate Students [2019YJSB104]
- National Cancer Institute [R01 CA175741]
- Elsa U. Pardee Foundation [R01 CA175741]
EHF plays a key role in decreasing the sensitivity of pancreatic cancer cells to CSCs' niche stimulus by suppressing the transcription of the CXCL12 receptor CXCR4. Rosiglitazone can target PC stem cells and inhibit the crosstalk between CSCs and their niche by upregulating EHF.
Background and aims The crosstalk between cancer stem cells (CSCs) and their niche is required for the maintenance of stem cell-like phenotypes of CSCs. Here, we identified E26 transformation-specific homologous factor (EHF) as a key molecule in decreasing the sensitivity of pancreatic cancer (PC) cells to CSCs' niche stimulus. We also explored a therapeutic strategy to restore the expression of EHF. Design We used a LSL-Kras(G12D/+)mice, LSL-Trp53(R172H/+) and Pdx1-Cre (KPC) mouse model and samples from patients with PC. Immunostaining, flow cytometry, sphere formation assays, anchorage-independent growth assay, in vivo tumourigenicity, reverse transcription PCR, chromatin immunoprecipitation (ChIP) and luciferase analyses were conducted in this study. Results CXCL12 derived from pancreatic stellate cells (PSCs) mediates the crosstalk between PC cells and PSCs to promote PC stemness. Tumorous EHF suppressed CSC stemness by decreasing the sensitivity of PC to CXCL12 stimulus and inhibiting the crosstalk between PC and CSC-supportive niches. Mechanically, EHF suppressed the transcription of the CXCL12 receptor CXCR4. EHF had a cell autonomous role in suppressing cancer stemness by inhibiting the transcription of Sox9, Sox2, Oct4 and Nanog. Rosiglitazone suppressed PC stemness and inhibited the crosstalk between PC and PSCs by upregulating EHF. Preclinical KPC mouse cohorts demonstrated that rosiglitazone sensitised PDAC to gemcitabine therapy. Conclusions EHF decreased the sensitivity of PC to the stimulus from PSC-derived CSC-supportive niche by negatively regulating tumorous CXCR4. Rosiglitazone could be used to target PC stem cells and the crosstalk between CSCs and their niche by upregulating EHF.
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