期刊
GENOMICS
卷 113, 期 3, 页码 1262-1271出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2020.10.042
关键词
Soybean; Sclerotinia stem rot; Single-nucleotide polymorphism; Genome-wide association study; Candidate genes
资金
- National Natural Science Foundation of China [31871650, 31971967]
- Heilongjiang Provincial Project [C2018016, JC2018007, GX17B002, GJ2018GJ0098]
- National Key Research and Development Project [2017YFD0101306, 2016YFD0100304, 2017YFD0101302]
- Youth Leading Talent Project of the Ministry of Science and Technology in China [2015RA228]
- National Ten Thousand Talents Program
- Postdoctoral Fund in Heilongjiang Province [LBHQ20004, LBH175]
- [2014BAD22B01]
- [2016ZX08004001007]
- [CARS-04-PS04]
The study of 185 representative soybean accessions revealed partial resistance related to SSR disease, and potential candidate genes were identified through sequencing and association analysis.
Sclerotinia stem rot (SSR) is a disease of soybean [Glycine max (L.) Merr] that causes severe yield losses. We studied 185 representative soybean accessions to evaluate partial SSR resistance and sequenced these by the specific-locus amplified fragment sequencing method. In total, 22,048 single-nucleotide polymorphisms (SNPs), with minor allele frequencies (MAF) >= 5% and missing data <3%, were developed and applied to genome-wide association study of SSR responsiveness and assess linkage disequilibrium (LD) level for candidate gene selection. We identified 18 association signals related to SSR partial resistance. Among them, six overlapped the regions of previous quantitative trait loci, and twelve were novel. We identified 243 candidate genes located in the 200 kb genomic region of these peak SNPs. Based on quantitative real-time polymerase chain reaction and haplotype analysis, Glyma.03G196000 and Glyma.20G095100, encoding pentatricopeptide repeat proteins, might be important factors in the resistance response of soybean to SSR.
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