期刊
GENE
卷 783, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.gene.2021.145553
关键词
Exercise training; Spermatogenesis; Metabolic interactions; GLUT-1; GLUT-3; MCT-4
资金
- Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University
- Departments of Basic Sciences, Urmia University
- Faculty of Veterinary Medicine, Urmia University
Low and moderate-intensity exercise training did not significantly affect the expression levels of metabolic substrates transmembrane transporters in testicular tissue, while high-intensity training led to suppression of protein contents of GLUT-1, GLUT-3, and MCT-4 in SCs and reduced SCs survival, resulting in failed spermatogenesis and spermiogenesis.
Despite other tissues, the effect of different exercise training protocols (ETPs) on the expression levels of metabolic substrates transmembrane transporters in the testicular tissue, remains completely unexplored. Thus, the effects of low, moderate and high-intensity ETPs on the SCs and germ cells potentials in GLUT-1, GLUT-3 and MCT-4 expression levels was investigated in this study. The animals were assigned into 4 groups, including sedentary control, low-intensity continuous (LICT), moderate-intensity (MICT) and high-intensity (HICT) ETPs-induced groups (n = 6/group). The GLUT-1, GLUT-3 and MCT-4 expressions, cytoplasmic carbohydrate storages of SCs and germ cells, the SCs survival and the spermatogenesis and spermiogenesis rates were assessed. The LICT and MICT did not significantly alter the protein expression levels of GLUT-3 and MCT-4 in the SCs and germ cells, while decreased the GLUT-1 protein content versus the sedentary control animals. In contrast, the HICT remarkably suppressed the GLUT-1 and MCT-4 in both SCs, and germ cells and diminished GLUT-3 in SCs and increased in the germ cells. No significant changes were revealed in the cytoplasmic carbohydrate storage in the LICT and MICT groups, while significantly diminished in the HICT group. The HICT group showed a failed spermatogenesis and spermiogenesis, which were not demonstrated in the sedentary control, LICT and MICT groups. In conclusion, the HICT, by reducing the GLUT-1, GLUT-3 and MCT-4 protein contents in the SCs and reducing the SCs survival, can suppress the glucose transmembrane transport and inhibit the lactate export from SCs, which in turn, ends with failed spermatogenesis and spermiogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据