期刊
GENE
卷 768, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.gene.2020.145321
关键词
c/ebp alpha; Transcription factor; Testis; dmrt1; Gonadal differentiation; Cynoglossus semilaevis
资金
- National Nature Science Foundation of China [31472269, 31722058, 31802275]
- National Key R&D Program of China [2018YFD0900301]
- Qingdao National Laboratory for Marine Science and Technology [2017ASTCP-ES06]
- Taishan Scholar Project Fund of Shandong of China
- National Ten-Thousands Talents Special Support Program
- Central Public-interest Scientific Institution Basal Research Fund, CAFS [2020TD19]
- International Scientific Partnership Program ISPP at King Saud University [0050]
The study showed that c/ebp alpha is highly expressed in the gonads of Chinese tongue sole and plays a crucial negative regulatory role in early gonadal differentiation during the juvenile period. Additionally, c/ebp alpha was found to negatively regulate the male-determining gene dmrt1 in vitro.
c/ebp alpha is a member of the C/EBP family of transcription factors, which are involved in cell growth and differentiation and have a conserved basic leucine zipper (bZIP) domain. However, little is known about its function in sex determination and differentiation. In the present study, c/ebp alpha was cloned from the gonads of Chinese tongue sole (Cynoglossus semilaevis). The full-length cDNA of c/ebp alpha was 1583 bp, with a 198-bp 5' UTR, a 446-bp 3' UTR, and a 939-bp open reading frame encoding a 312-amino acid peptide. qRT-PCR revealed that c/ebp alpha was predominantly expressed in undifferentiated gonads of male C. semilaevis at 30 dpf and 60 dpf and peaked at 60 dpf. Expression levels of c/ebp alpha in the testis were constantly higher than those in ovaries at all developmental stages. Moreover, a dual-luciferase assay revealed that c/ebp alpha could negatively regulate the male-determining gene dmrt1 in vitro. These results provide fundamental information indicating that C. semilaevis c/ebp alpha might be involved in early gonadal differentiation and functions as a negative regulator of dmrt1 by repressing its transcription.
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