期刊
FUNCTIONAL & INTEGRATIVE GENOMICS
卷 21, 期 2, 页码 205-214出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10142-021-00770-3
关键词
Placenta; Single-cell RNA-seq; Transcription factor; Trophectoderm
资金
- Young scientist startup grand of The Second Affiliated Hospital of Zhengzhou University
- Foundation of Henan Educational Committee (CN) [19A320044]
This study reanalyzed single-cell RNA-seq data from post-implantation embryos derived from different sources, identifying different subtypes of trophoblast cells and their characteristic markers, as well as investigating the gene expression patterns of trophoblast-specific transcription factors in different models.
The dysfunction of placenta development is correlated to the defects of pregnancy and fetal growth. The detailed molecular mechanism of placenta development is not identified in humans due to the lack of material in vivo. Trophoblast (TB) lineage derived from human embryonic stem cells (hESCs) induced by bone morphogenetic protein 4 (BMP4) has been applied as a model for studying TB lineage specification in vitro. With the development of single-cell sequencing technology, it became possible to detect the transcriptome of the post-implantation embryo at unprecedented precision. In this study, we reanalyzed single-cell RNA-seq of post-implantation embryos derived from two separate groups and identified different subtypes of trophoblast cells and their marker, respectively. At the same time, we focused on the gene expression patterns of trophoblast-specific transcription factors in different models. Our analysis sheds new light on the transcription regulation mechanism of trophoblast differentiation at the early stage of pregnancy establishment in human.
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