期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 164, 期 -, 页码 399-409出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2021.01.027
关键词
Superoxide dismutase 3 (SOD3); Zebrafish; Bone homeostasis; Redox regulation; Bone mineralization
资金
- Aarhus University Graduate School
- Independent Research Fund Denmark - Medical Sciences [DFF-7016-00188]
- Beckett Foundation
The study revealed that SOD3 plays an unappreciated role in bone development and homeostasis, with Sod3b showing specific expression in skeletal tissue in zebrafish.
Superoxide dismutase 3 (SOD3) is an extracellular protein with the capacity to convert superoxide into hydrogen peroxide, an important secondary messenger in redox regulation. To investigate the utility of zebrafish in functional studies of SOD3 and its relevance for redox regulation, we have characterized the zebrafish orthologues; Sod3a and Sod3b. Our analyses show that both recombinant Sod3a and Sod3b express SOD activity, however, only Sod3b is able to bind heparin. Furthermore, RT-PCR analyses reveal that sod3a and sod3b are expressed in zebrafish embryos and are present primarily in separate organs in adult zebrafish, suggesting distinct functions in vivo. Surprisingly, both RT-PCR and whole mount in situ hybridization showed specific expression of sod3b in skeletal tissue. To further investigate this observation, we compared femoral bone obtained from wild-type and SOD3(-/-) mice to determine whether a functional difference was apparent in healthy adult mice. Here we report, that bone from SOD3(-/-) miceis less mineralized and characterized by significant reduction of cortical and trabecular thickness in addition to reduced mechanical strength. These analyses show that SOD3 plays a hitherto unappreciated role in bone development and homeostasis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据