期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 163, 期 -, 页码 243-254出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2020.12.019
关键词
Cytochrome bc(1); aa(3) oxidase; Superoxide anion radical; Reactive oxygen species; Mitochondrial mutations; Amplex red
资金
- Opus grant [2016/21/B/NZ1/00294]
- National Science Center Poland
Cytochrome bc(1), also known as mitochondrial complex III, is an important producer of ROS in living organisms; recent research shows that it can also generate ROS in a non-inhibited system and that the production is influenced by the rate of operation and mitochondrial mutations.
Cytochrome bc(1), also known as mitochondrial complex III, is considered to be one of the important producers of reactive oxygen species (ROS) in living organisms. Under physiological conditions, a certain level of ROS produced by mitochondrial electron transport chain (ETC) might be beneficial and take part in cellular signaling. However, elevated levels of ROS might exhibit negative effects, resulting in cellular damage. It is well known that inhibiting the electron flow within mitochondrial complex III leads to high production of ROS. However, superoxide production by cytochrome bc(1) in a non-inhibited system remained controversial. Here, we propose a novel method for ROS detection in ETC hybrid system in solution comprising bacterial cytochrome bc(1) and mitochondrial complex IV. We clearly show that non-inhibited cytochrome bc(1) generates ROS and that adaptive and pathogenic mitochondrial mutations suppress and enhance ROS production, respectively. We also noted that cytochrome bc(1) produces ROS in a rate-dependent manner and that the mechanism of ROS generation changes according to the rate of operation of the enzyme. This dependency has not yet been reported, but seems to be crucial when discussing ROS signaling originating from mitochondria.
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