4.7 Article

Activation of caspase-3 in gastric adenocarcinoma AGS cells by Xylopia aethiopica (Dunal) A. Rich. fruit and characterization of its phenolic fingerprint by HPLC-DAD-ESI(Ion Trap)-MSn and UPLC-ESI-QTOF-MS2

期刊

FOOD RESEARCH INTERNATIONAL
卷 141, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.foodres.2021.110121

关键词

Acacetin glycosides; Apigenin glycosides; African pepper; Guinea pepper; Kaempferol glycosides; Luteolin glycosides; Quercetin glycosides

资金

  1. FCT/MCTES [UIDB/50006/2020]
  2. Programa de Cooperacion Interreg V-A Espana - Portugal (POCTEP) 2014-2020 [0377_IBERPHENOL_6_E]
  3. COMPETE 2020 program
  4. FEDER
  5. European Union [PTDC/ASP-AGR/30154/2017 (POCI-01-0145-FEDER-030154)]

向作者/读者索取更多资源

The study investigated the phenolic composition of Xylopia aethiopica fruits and found potent cytotoxic effects against cancer cells, particularly towards AGS cells. The extract induced apoptosis and activated caspase-3, shedding light on the anticancer mechanisms of X. aethiopica fruits. Further research is needed to identify non-phenolic constituents contributing to these effects and to detail the anticancer mechanisms.
While the fruits of Xylopia aethiopica (Dunal) A. Rich. are important in African countries as a local trade product, their composition remains scarcely investigated. Phenolic fingerprint is herein delivered through HPLC-DAD-ESI (Ion Trap)-MSn and UPLC-ESI-QTOF-MS2 analysis, six cinnamoylquinic acid derivatives and twenty-four flavonoid glycosides being determined, chrysoeriol-7-O-glycosides being the main constituents. A cytotoxicity screening of twenty-eight hydroethanol extracts, obtained from a collection of Guinea-Bissauan plants, against A549 and AGS carcinoma cells, revealed the selective and potent effect towards AGS cells (IC50 = 151 x 10(-3) g L-1), upon exposure to the extract from X. aethiopica fruits. Additional experiments demonstrated insignificant effect on LDH release at 151 x 10(-3) g L-1, morphological analysis further suggesting induction of apoptosis. Pro-apoptotic effects were confirmed, as the extract enabled the activation of the effector caspase-3, broadening the knowledge on the anticancer mechanisms elicited by the fruits of X. aethiopica. Phenolic constituents might contribute to the cytotoxic effects, particularly via caspase-3 activation. Considering that X. aethiopica fruit is very often referred as an anticancer ingredient in Africa, but mainly the potent cytotoxicity herein recorded, our results call for additional research aiming to identify non-phenolic constituents contributing to the effects and also to further detail the anticancer mechanisms.

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