期刊
FOOD RESEARCH INTERNATIONAL
卷 140, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.foodres.2020.110067
关键词
Arabinoxylan; Immunomodulatory; Nuclear factor-kappa B; Transcriptome analyses
资金
- National Natural Science Foundation of China [31700304]
- Natural Science Foundation of Jiangsu Province [BK20201480]
This comprehensive transcriptional study on wheat bran extracted arabinoxylan (AX) revealed significant impact on immune-related genes and pathways, providing valuable information for further research on the complex molecular mechanisms associated with AX. The study identified 2325 differentially expressed genes (DEGs), mainly enriched in immune-related processes, with top pathways including NF-kappa B and TNF signaling pathways. Additionally, AX treatment led to dynamic changes in mRNA levels of immune-related genes and up-regulation of NF-kappa B nuclear translocation and upstream target proteins.
Arabinoxylan (AX) extracted from wheat bran has attracted much attention due to its immunomodulatory activity. However, the molecular mechanisms underlying this activity remain unclear. In this study, we conducted a comprehensive transcriptional study to investigate genetic changes related to AX and identified 2325 differentially expressed genes (DEGs). Gene Ontology classification revealed that the DEGs were mainly enriched in a series of immune-related processes. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that immune-related pathways were significantly enriched in top 20 pathways, including the nuclear factor-kappa B (NF-kappa B) signaling pathway and the TNF signaling pathway. Validation using quantitative polymerase chain reaction analysis revealed dynamic changes in the mRNA levels of immune-related Cd40, Csf1, Csf2, Fas, IL-1 beta, IL 6, IL-5, Irf1, and Tnfaip3, which were significantly up-regulated in the AX-treated group. Moreover, AX treatment led to the up-regulation of the nuclear translocation of NF-kappa B and its upstream target proteins such as PDK1, Akt, I kappa B-alpha, and GSK-3 beta. The dataset compiled from this study provides valuable information for further research on the complex molecular mechanisms associated with AX and the identification of target genes.
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