期刊
FOOD CONTROL
卷 121, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2020.107664
关键词
Sample concentration; LAMP; Magnetic bead; Aptamer; Pt/Pd nanoparticle; Food safety
资金
- Iranian Ministry of Science and research Council of University of Tehran [28645/01/02]
- Horizon 2020 framework program of the European Union under the project VIVALDI [773422]
- H2020 Societal Challenges Programme [773422] Funding Source: H2020 Societal Challenges Programme
The study developed a catalytic nano-bioprobe immobilized with a Salmonella-specific DNA aptamer on magnetic beads, enabling rapid detection of low levels of Salmonella enterica serovar Typhimurium in food and chicken fecal samples; in less than 3 hours, as low as 10-15 CFU/mL of the bacteria could be captured and detected with the combination of loop mediated isothermal amplification (LAMP) technology.
Concentration of pathogens directly from food samples by using magnetic beads is a potential strategy in the on site food sample analysis. In this study, magnetic beads, double modified with platinum/palladium nanoparticle (Pt/Pd NP) and DNA aptamer, is presented as catalytic nano-bioprobes for the on-site detection of Salmonella enterica serovar Typhimurium in food and fecal samples. Combination of the developed catalytic nano-bioprobes with loop mediated isothermal amplification (LAMP) enabled rapid detection of low levels of S. Typhimurium in food and chicken fecal samples without culture enrichment. S. Typhimurium-specific DNA aptamer immobilized on the magnetic bead could efficiently concentrate S. Typhimurium with a capturing efficiency higher than 76% in phosphate-buffered saline (PBS). Further, DNA-mediated inhibition of peroxidase-mimic activity of Pt/Pd NP in combination with LAMP was used as a unique approach to detect S. Typhimurium. With this unique approach, it was possible to capture and detect S. Typhimurium as low as 10-15 CFU/mL in chicken meat sample and 3-10 CFU/mL in both whole egg and chicken fecal samples within less than 3 h. Analysis of S. Typhimurium-spiked chicken meat, whole egg and chicken fecal materials have confirmed the precision of the method. A relative accuracy of 90% with an intra and inter assay precision of 8.36% and 9.92% respectively was achieved in the S. Typhimurium-spiked food samples. This unique approach has the potential for the integration in to Lab-on-a-chip based biosensors for on-site monitoring of foodborne pathogens in future.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据