Exploration of biodegradation mechanism by AFB1-degrading strain Aspergillus niger FS10 and its metabolic feedback

Significant progress has been made in the biological degradation of aflatoxin B-1 (AFB(1)), which is the most toxic and carcinogenic mycotoxin commonly found in food and feed products; however, research in this area has currently focused on the effective methods for detoxifying AFB(1) and the mechanisms underlying the degradation process. The relationship between AFB(1) biotransformation and the metabolic feedback by the AFB(1)-degrading strain has rarely been reported. This study investigated the biotransformation pathway of AFB(1) in a highly efficient degrading strain, Aspergillus niger FS10, and the metabolic regulation of FS10 during AFB(1) degradation. We analyzed the AFB(1) metabolic degradation products and evaluated its toxicity based on its structures by triple quadrupole-linear ion trap-mass spectrometry (Q-Trap-MS) coupled with LightSight (TM) Software. Four metabolic degradation products were obtained: Product 1 (C17H14O6), Product 2 (C17H14O7), Product 3 (C16H12O5), and Product 4 (C27H31N3O13, AFB(2)-GOH). Two biotransformation pathways were proposed based on the structures of these degradation products. Metabolomics data revealed that FS10 exhibited different degrees of bioremediation under low-, mediumand highdose AFB(1). The results also suggested the significant involvement of glutathione, a metabolite, in the AFB(1) biotransformation pathway and its formation of AFB(2)-GOH with AFB(1). The four products could probably be less toxic than AFB(1), as reflected by changes in the AFB(1) toxicity site. Therefore, the AFB(1)-degrading strain FS10 exhibits great potential for application in the detoxification of AFB(1) in food and feed products.

Automated summary

This study investigated the biotransformation pathway of AFB(1) in Aspergillus niger FS10 and its metabolic regulation during AFB(1) degradation. Four metabolic degradation products were identified, leading to the proposal of two biotransformation pathways. Metabolomics data showed that FS10 exhibited varying degrees of bioremediation under different doses of AFB(1), with glutathione playing a significant role in the biotransformation process.