期刊
FOOD CHEMISTRY
卷 339, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128059
关键词
Kanamycin; Aptamer; Nicking enzyme; Recycling amplification; Colorimetric biosensor
资金
- Fundamental Research Funds for the Central Universities [GK201802012]
- Key Research and Development Plan of Shaanxi Province [2020SF-374]
A colorimetric aptasensing strategy for detection of kanamycin was developed based on aptamer biorecognition and signal amplification with nicking enzyme, showing good selectivity and sensitivity with a detection limit as low as 0.2 pg/mL. The method can potentially be extended for the detection of other antibiotics in foods by adapting corresponding aptamer sequences.
A colorimetric aptasensing strategy for detection of kanamycin was designed based on aptamer biorecognition and signal amplification assisted by nicking enzyme. The aptamer of kanamycin was designed to be contained in the metastable state hairpin DNA. The target DNA as recycling DNA was located in the loop of hairpin DNA. The presence of kanamycin stimulates the continuous actions, including specific recognition of the aptamer to kanamycin, the hybridization between target DNA and signal probe, the cleavage function of nicking enzyme. The actions induced accumulation of numerous free short sequences modified by platinum nanoparticles (PtNPs), which can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB)-H2O2 to produce a colorimetric response. The aptasensor exhibited good selectivity and sensitivity for kanamycin in milk with a detection limit as low as 0.2 pg.mL(-1). In addition, the proposed assay is potentially to be extended for other antibiotics detection in foods by adapting the corresponding aptamer sequence.
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