Identification of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides from vegetable protein sources

Vegetable proteins are appearing as a sustainable source for human consumption. Food-derived peptides are an important field of research in terms of bioactive molecules. In this study, seven vegetable proteins were enzymatically hydrolysed following an optimised treatment (sequential hydrolysis with subtilisin-trypsinflavourzyme) to obtain dipeptidyl peptidase IV (DPP-IV) inhibitory peptides. Hydrolysates were fractionated by size exclusion chromatography and, from the most bioactive fractions (corresponding to Glycine max, Chenopodium quinoa and Lupinus albus proteins); peptides responsible for this bioactivity were identified by mass spectrometry. Peptides with adequate molecular features and based on in silico analysis were proposed as DPP-IV inhibitors from soy (EPAAV) lupine (NPLL), and quinoa (APFTVV). These vegetable protein sources are adequate to obtain protein hydrolysates for functional food.

Automated summary

This study focused on enzymatically hydrolysing seven vegetable proteins to obtain peptides with DPP-IV inhibitory activity. The most bioactive peptides were identified from soy, quinoa, and lupine proteins using mass spectrometry. Peptides from these vegetable protein sources show potential as DPP-IV inhibitors for functional food applications.