期刊
FOOD CHEMISTRY
卷 354, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129536
关键词
Immunosensor; Arginine kinase; FRET; Antigen?antibody interaction
资金
- National Natural Science Foundation of China [31871735]
- Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholars of China [LR19C200001]
A novel immunoassay for quantitation of shrimp arginine kinase (AK) was developed using gold nanoparticles and quantum dots, showing high sensitivity and specificity.
Arginine kinase (AK) is one of the most important allergens in shrimp products. Herein, a novel immunoassay for quantitation of AK was developed using the antibody modified gold nanoparticle (AuNP) and quantum dot (QD). When the first antibody modified AuNP (AuNP-Ab1) was bridged by AK with the secondary antibody modified QD (QD-Ab2), fluorescence resonance energy transfer (FRET) would occur between the AuNP and QD, which led to a decrease in fluorescent signals. The decrease in fluorescence intensity was found to correlate linearly with the log of AK concentration in the range of 1.0 ? 10-6-1.0 ? 10-3 mg/mL (R2 = 0.9909) and the detection limit was 0.11 ng/mL. The immunoassay was further proved to have encouraging specificity, precision and accuracy. Compared with existing methods, this study provided a promising approach to develop a highly sensitive and selective detection method for AK in shrimp related food samples.
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