期刊
FOOD CHEMISTRY
卷 339, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.127854
关键词
Lateral flow assay; Metal-polydopamine framework; Tetracycline; Food analysis
资金
- Talented Program [A279021724]
- Fundamental Research Funds for the Northwest AF University [Z111021601]
By employing metal-polydopamine framework as a label and using a versatile layer-by-layer assembly strategy, a highly sensitive biosensor for tetracycline detection was developed. The biosensor showed a strong linear relationship, low detection limit, and an increased sensitivity of over 66-fold compared to traditional AuNPs-based LFA.
Gold nanoparticles (AuNPs)-based lateral flow assay (LFA) enables a rapid detection of tetracycline (TET) in food samples but suffers from low sensitivity. Herein, metal-polydopamine framework (MPF), as a label, was employed to load monoclonal antibodies (mAbs) directly as the probe in LFA for highly sensitive sensing of TET. Combining zeolitic imidazolate framework (ZIF-67) and polydopamine (PDA), a stable MPF with large size, well water-dispersible, excellent affinity and optical properties was prepared through a versatile layer-by-layer assembly (LLA) strategy. Under optimized conditions, the biosensor (MPF-LFA) exhibited a great linear relationship in the range of 0.09-6 ng/mL and a detection limit of 0.045 ng/mL for TET detection, which was over 66-fold more sensitive than traditional AuNPs based LFA. Furthermore, the MPF-LFA was successfully applied to the screening of TET in fish, chicken, milk and shrimp samples with satisfied recoveries from 91% to 114%.
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