期刊
FOOD CHEMISTRY
卷 353, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129521
关键词
Distilled spent grain (DSG); Baijiu (Chinese liquor); ACE inhibitory peptides; Proteomics; Molecular docking
资金
- National Key R&D Program of China [2016YFD0400503]
- national first-class discipline program of Light Industry Technology and Engineering [LITE2018-12]
This study developed a new method for identifying bioactive peptides, successfully identifying 22 peptides with ACE inhibitory activities, providing promising candidates for ACE inhibition in DSG.
Distilled spent grain (DSG) is the biggest by-product in baijiu (Chinese liquor) production, releasing approximately 23.44 million tons every year. Aiming at comprehensive identification of more bioactive peptides, in this work, the new bioassay-guided proteomics and Biolynx peptide sequencer based on ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) were developed. Moreover, 22 peptides with angiotensin converting enzyme (ACE) inhibitory activities were identified. Seven peptides were successfully quantified using electrospray ionization with triple-quadrupole mass spectrometry (ESI-QQQ-MS) in the multiple reaction monitoring (MRM). Of these identified peptides, Pro-Arg was the most abundant (92.14 mu g g-1 dry weight (DW)) and acted as a competitive inhibitor of ACE by molecular docking. Therefore, peptides from DSG can be considered as promising candidates for ACE inhibition; in addition, the new strategy for peptide sequencing can be extended to any food matrices containing peptide mixture or protein hydrolysate.
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