Structurally diverse coumarin-homoisoflavonoid derivatives with hepatoprotective activities from the fruits of Cucumis bisexualis

Cucumis bisexualis is a favorite wild fruit with high nutritional and medicinal values because of its bioactive constituents. Four previously undescribed coumarin-homoisoflavonoid derivatives (1-4), together with seven known coumarin and homoisoflavonoid derivatives (5-11) were isolated from the fruits of C. bisexualis for the first time. All the compounds were elucidated by their extensive and comprehensive spectroscopic data and references. Compounds (1-11) were evaluated for their hepatoprotective activities in HepG2 cells by the acetaminophen (APAP)-induced damage model at 10.0 mu M with bicyclol as the positive control. Among them, compounds 1, 3, 5, and 6 showed moderately hepatoprotective activities to improve the HepG2 cell survival rates from 51.68 +/- 2.49% (APAP, 10 mM) to 71.55 +/- 4.08%, 65.95 +/- 4.39%, 60.77 +/- 3.44%, 62.94 +/- 2.30%, respectively.

Automated summary

Cucumis bisexualis is a wild fruit with high nutritional and medicinal values. Several previously undescribed compounds isolated from its fruits show moderate hepatoprotective activities.