期刊
FISH & SHELLFISH IMMUNOLOGY
卷 109, 期 -, 页码 41-50出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2020.11.022
关键词
Hemocytes; Nitric oxide; Butaclamol; Tetralin; SKF-38393
资金
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Institute of Biodiversity and Sustainability (NUPEM)
- Foundation Carlos Chagas Filho of the Research Support of the State of Rio de Janeiro (FAPERJ) [E-26/203.021/2018]
DA, an important neurotransmitter, can modulate NO production in immune cells through adrenergic receptors in ascidian animals. The presence of catecholaminergic receptors on ascidian immune cells suggests unique pharmacological characteristics that may have evolutionary significance.
Dopamine (DA) is an important molecule that plays a role in the nervous and immune systems. DA is produced by a wide variety of animals and it is considered one of the oldest neurotransmitters. However, its specific function in immune cells has not been completely revealed. In a group of chordate animals, the ascidians, DA is reported to be produced by cells in the central nervous system (CNS); however, no dopaminergic receptor in their genomes has been described until now. Because this is an integrating characteristic of the ascidian dopamine system, here it was investigated the pharmacology, function, and phylogeny of DA and dopaminergic receptors (DRs) in the modulation of nitric oxide (NO) in the Phallusia nigra immune cells. The data disclosed, for the first time, that DA modulates NO production by immune cells. Its modulation probably occurs though adrenergic receptors, which display a special characteristic, in that they are capable of binding to noradrenaline (NA) and DA. A pharmacological analysis revealed that receptors present on the ascidian immune cells showed a high affinity to butaclamol, a non-selective D2-class receptor, increasing NO production. In addition, calcium intracellular mobilization was observed when DA was added to immune cells. In conclusion, the data revealed novel insights about the presence of catecholaminergic receptors (CRs) on the P. nigra immune cells, indicating that ascidian CRs have special pharmacological characteristics that are worth highlighting from an evolutionary point of view.
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