期刊
FASEB JOURNAL
卷 35, 期 3, 页码 -出版社
WILEY
DOI: 10.1096/fj.202001970R
关键词
adipokine; canonical correlation analysis; collagen; fibrotic; metabolic syndrome; scRNA‐ seq
资金
- HHS \ National Institutes of Health (NIH) [AG15768, AG46927]
This study identified 11 unique myeloid and myeloid-associate cell populations in mouse adipose tissue through single-cell RNA sequencing, immunofluorescence, and flow cytometry. The research revealed changes in various cell populations in obese mice and identified a novel myeloid/macrophage population with pro-fibrotic characteristics.
Macrophages and other immune cells are important contributors to obesity-associated inflammation; however, the cellular identities of these specific populations remain unknown. In this study, we identified individual populations of myeloid cells found in mouse epididymal/visceral adipose tissue by single-cell RNA sequencing, immunofluorescence, and flow cytometry. Multiple canonical correlation analysis identified 11 unique myeloid and myeloid-associate cell populations. In obese mice, we detected an increased percentage of monocyte-derived pro-inflammatory cells expressing Cd9 and Trem2, as well as significantly decreased percentages of multiple cell populations, including tissue-resident cells expressing Lyve1, Mafb, and Mrc1. We have identified and validated a novel myeloid/macrophage population defined by Ly6a expression, exhibiting both myeloid and mesenchymal characteristics, which increased with obesity and showed high pro-fibrotic characteristics in vitro. Our mouse adipose tissue myeloid cell atlas provides an important resource to investigate obesity-associated inflammation and fibrosis.
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