期刊
EXPERIMENTAL EYE RESEARCH
卷 204, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2021.108443
关键词
Hydrogen sulfide; Retinal artery; Retina; 3-MST; CBS; CSE
资金
- TUBITAK (Scientific and Technological Research Council of Turkey) [115S196]
- Scientific Research Projects Coordination Unit of Istanbul University [TDK-2018-29789]
- Scientific Research Projects Coordination Unit of Giresun University [SAG-BAP-A-230218-81]
The study investigated the concentration levels and distribution profiles of H2S synthesizing enzymes in the bovine retina and retinal artery. CBS and CSE were found to have higher concentrations in the retinal artery compared to the retina, with 3-MST at the lowest level. The distribution of these enzymes also varied, with CBS found in endothelial and smooth muscle layers, while CSE was mainly present in the endothelial layer of the retinal artery.
Hydrogen sulfide (H2S) is an important gasotransmitter expressed in various tissues of the organism, including the eye. It is known that H2S is localized especially in the retina and corneal layers in bovine eye. The enzymes that mediate H2S synthesis are 3-mercaptopyruvate sulfurtransferase (3-MST), cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE). Herein, we aimed to investigate the concentration levels and distribution profiles of these enzymes in bovine retina and retinal artery. Enzyme levels were measured by ELISA and distribution were determined by immunofluorescence microscopic analysis. Much higher concentrations of CBS and CSE have been detected in the retinal artery compared to the retina. In both tissues, particulary 3-MST was found at the lowest level while, CSE was determined to be the most abundant enzyme among the others. CBS distribution was shown in both endothelial and smooth muscle layers, while CSE was seen especially in the endothelial layer of the retinal artery. In the retina, CBS and CSE were expressed in cone-basil cells and retinal ganglion cells, while CSE was also present in bipolar cells. Our results indicated that H2S is synthesized endogenously in ocular tissues. The widespread expression of H2S synthesizing enzymes in the retina and retinal artery of the bovine eye, which has anatomical similarities with the human eye, may suggest a protective role for H2S against retinal vascular diseases as well as a regulatory role in the retinal vascular tone.
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