期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 144, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.enzmictec.2020.109728
关键词
Sepsis; Diagnosis; Phospholipid; Enzymatic assay; Cyclic voltammetry; Graphite
资金
- National Research Foundation of Korea [NRF-2020R1A2B5B01002187, NRF-2020R1A5A101913111]
In this study, phospholipid quantification was achieved using an enzymatic assay based on cyclic voltammetry, applied to the diagnosis of sepsis and compared with the conventional method of optical density measurement.
In this work phospholipid quantification was carried out using an enzymatic assay based on cyclic voltammetry of the condensation product of N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline sodium salt (DAOS) and 4-aminoantipyrine (4-AP) with a graphite electrode. For the optimization of electrochemical measurement for the product, electrochemical properties such as the electrochemical window, double layer capacitance (Cdl) and electron transfer rate (kapp) were analyzed for a graphite-electrode and Au-electrode. The phospholipid enzymatic assay based the on electrochemical measurement using the graphite electrode was applied to the diagnosis of sepsis for sera from healthy volunteers (n = 16), patients with systemic inflammatory response syndrome (SIRS, n = 16) and severe sepsis patients (n = 24). Finally, the phospholipid quantification results from the electrochemical measurement were statistically compared with the conventional method based on optical density measurement.
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