Control of red flour beetle (Tribolium castaneum) in feeds and commercial poultry diets via using a blend of clove and lemongrass extracts

The insects' infestation decreases the nutritive value of the stored grains and causes losses in its weight, quality, or economic values. The aim of the present study was to investigate the efficiency of a natural product of clove and lemongrass extracts in controlling of red flour beetle (Tribolium castaneum) in different feedstuffs and commercial poultry diets. Different concentrations of the tested product (5, 10, 20, 50, 100 mg) were mixed with 10 g of different feedstuffs and poultry diets, and incubated at different time points. The percent repellency (PR), toxicity effect, and antifeedant activity of the plant extracts compound were assessed. The PR was detected depending on the choice method. It was found that the PR was dose and time dependent. The highest doses (50 or 100 mg/10 g feed) achieved the highest repellency effect reaching 70% at 24 h post-application (PA). The minimal PR was reported by the lowest dose of 5 mg/10 g feed reaching 50% at 24 h PA. Moreover, the PR was found to decrease by time. The toxicity effect of this natural product on T. castaneum was cumulative, not acute, in which it was recorded after 1 month of application. Besides, this effect needs high doses of the product (at dose 500 or 1000 mg/50 g feed). The used product achieved a clear antifeedant activity against T. castaneum, as the feeding deterrent index (FDI %) for corn grains was 98.5% at 1.0 and 2.0% concentration, whereas for wheat grains there was a significant difference between both concentration (96.0 vs. 74.4%). In addition, the weight loss of control corn and wheat grains was higher than the treated ones, and it was about 3.15% and 2.0% per month for corn and wheat, respectively. In conclusion, the clove and lemongrass extracts had a repellency effect reaching to 70%. Moreover, it had a lethal effect on T. castaneum. In addition, it can reduce the weight loss of the infested feeds and consequently increasing its FDI %. Therefore, the clove and lemongrass extract blend can be used to protect the feedstuffs from the damage by this insect.

Automated summary

The use of clove and lemongrass extracts can effectively control red flour beetles, with repellent, toxic effects, and antifeedant activity, reducing weight loss in feeds and increasing FDI %.