期刊
ENVIRONMENTAL POLLUTION
卷 279, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2021.116942
关键词
Transcriptomic; Metabolomics; Toxic algal bloom; Zooplankton
资金
- National Science Foundation of China [41606131, 41576120]
- State Environmental Protection Key Laboratory of Coastal Ecosystem [201812]
- Dalian University of Technology via the Fundamental Research Funds for the Central Universities [DUT17RC(4)37]
- Canada First Research Excellence Fund [419205]
- Fisheries and Oceans Canada's National Contaminants Advisory Group
- Canada Research Chairs Program of the Natural Sciences and Engineering Research Council of Canada (NSERC)
- H2020-MSCA-IF-2018 fellowship [839243]
- CETOCOEN PLUS project of the ESIF [CZ.02.1.01/0.0/0.0/15_003/0000469]
- RECETOX research infrastructure [LM2018121]
- Banting post-doctoral fellowship
- Department of Biology, University of Southern Denmark
Exposure to Prorocentrum lima can lead to lethality in the brine shrimp Artemia salina and induce detoxification responses to oxidative stress. Protein degradation and energy expenditure are considered as response mechanisms to exposure to P. lima. Exposure to okadaic acid may disrupt the molting and reproduction activities of brine shrimp.
Prorocentrum lima is a dinoflagellate that forms hazardous blooms and produces okadaic acid (OA), leading to adverse environmental consequences associated with the declines of zooplankton pop-ulations. However, little is known about the toxic effects and molecular mechanisms of P. lima or OA on zooplankton. Here, their toxic effects were investigated using the brine shrimp Artemia salina. Acute exposure of A. salina to P. lima resulted in lethality at concentrations 10 0-fold lower than densities observed during blooms. The first comprehensive results from global transcriptomic and metabolomic analyses in A. salina showed up-regulated mRNA expression of antioxidant enzymes and reduced non-enzyme antioxidants, indicating general detoxification responses to oxidative stress after exposure to P. lima. The significantly up-regulated mRNA expression of proteasome, spliceosome, and ribosome, as well as the increased fatty acid oxidation and oxidative phosphorylation suggested the proteolysis of damaged proteins and induction of energy expenditure. Exposure to OA increased catabolism of chitin, which may further disrupt the molting and reproduction activities of A. salina. Our data shed new in-sights on the molecular responses and toxicity mechanisms of A. salina to P. lima or OA. The simple zooplankton model integrated with omic methods provides a sensitive assessment approach for studying hazardous algae. (c) 2021 Elsevier Ltd. All rights reserved.
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