期刊
ENVIRONMENTAL POLLUTION
卷 271, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2020.116337
关键词
Microplastics; Dark-field hyperspectral microscopy; Polystyrene; Caenorhabditis elegans; Minimum detection size
资金
- Kazan Federal University [0671-2020-0058]
- Russian Federation presidential grant [MD-2153.2020.3]
This study introduces a novel methodology for visualizing and identifying nano- and microscale polymer particles, showcasing the potential of dark-field hyperspectral microscopy in detecting and quantifying microplastics pollution.
Microplastics pollution is a serious ecological threat, severely affecting environments and human health. Tackling microplastics pollution requires an effective methodology to detect minute polymer particles in environmental samples and organisms. Here were report a novel methodology to visualise and identify nanoscale (down to 100 nm) and microscale synthetic commercially-available uniform spherical polymer particles using dark-field hyperspectral microscopy in visible-near infrared (400-1000 nm) wavelength range. Polystyrene particles with diameters between 100 nm-1 mu m, polymethacrylate 1 mu m and melamine formaldehyde 2 mu m microspheres suspended in pure water samples were effectively imaged and chemically identified based on spectral signatures and image-assisted analysis. We succeeded in visualisation and spectral identification of pure and mixed nano- and microplastics in vivo employing optically-transparent Caenorhabditis elegans nematodes as a model to demonstrate the ingestion and tissue distribution of microplastics. As we demonstrate here, dark-field hyperspectral microscopy is capable for differentiating between chemically-different microplastics confined within live invertebrate intestines. Moreover, this optical technology allows for quantitative identification of microplastics ingested by nematodes. We believe that this label-free non-destructive methodology will find numerous applications in environmental nano- and microplastics detection and quantification, investigation of their biodistribution in tissues and organs and nanotoxicology. (C) 2020 Elsevier Ltd. All rights reserved.
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