期刊
ENVIRONMENTAL POLLUTION
卷 277, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2021.116818
关键词
Mycotoxin; Cell toxicity; Intestinal cell renewal; Cell proliferation; Cytotoxicity; Intestinal barrier function
资金
- Genofood ANR project [19CE34]
- China Scholarship Council
This study demonstrated that proliferative intestinal epithelial cells are more susceptible to the mycotoxin deoxynivalenol (DON) compared to differentiated cells. DON was also found to delay the differentiation process in the intestinal epithelium. The differential gene expression analysis revealed the involvement of various pathways in the response to DON and the differentiation process.
The intestinal epithelium is a functional and physical barrier formed by a cell monolayer that constantly differentiates from a stem cell in the crypt. This is the first target for food contaminants, especially mycotoxins. Deoxynivalenol (DON) is one of the most prevalent mycotoxins. This study compared the effects of DON (0-100 mu M) on proliferative and differentiated intestinal epithelial cells. Three cell viability assays (LDH release, ATP content and neutral red uptake) indicated that proliferative Caco-2 cells are more sensitive to DON than differentiated ones. The establishment of transepithelial electrical resistance (TEER), as a read out of the differentiation process, was delayed in proliferative cells after exposure to 1 mu M DON. Transcriptome analysis of proliferative and differentiated exposure to 0-3 mu M DON for 24 h revealed 4862 differentially expressed genes (DEG) and indicated an effect of both the differentiation status and the DON treatment. KEGG enrichment analysis indicated involvement of metabolism, ECM receptors and tight junctions in the differentiation process, while ribosome biogenesis, mRNA surveillance, and the MAPK pathway were involved in the response to DON. The number of differentially expressed genes and the amplitude of the effect were higher in proliferative cells exposed to DON than that in differentiated cells. In conclusion, our study shows that proliferative cells are more susceptible than differentiated ones to DON and that the mycotoxin delays the differentiation process. (C) 2021 Elsevier Ltd. All rights reserved.
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