Regulation of zinc homeostatic genes by environmental pH in the filamentous fungus Aspergillus fumigatus

Aspergillus fumigatus can grow over a broad range of pH values even though zinc availability is greatly conditioned by ambient pH. It has been previously shown that regulation of zinc homeostatic genes in this fungus relies on the transcription factor ZafA. In addition, their expression is further modulated by the transcription factor PacC depending on ambient pH, which allows this fungus to grow in diverse types of niches, including soils and the lungs of immunosuppressed hosts. In this work the regulation by PacC of genes zrfB and zrfC that are expressed, respectively, under acidic and alkaline zinc-limiting conditions have been analysed in detail. Thus, data that extend the current model for PacC function, including the role of the full-length PacC(72) protein and the PacC processed forms (PacC(53) and PacC(27)) on gene expression has been provided, and a new mechanism for the repression of acid-expressed genes in alkaline media based on interference with the start of transcription has been described. Moreover, it was proposed that the transcription of both acid-expressed and alkaline-expressed genes under zinc-limiting conditions might also rely on a third factor (putatively Pontin/Reptin), which may be required to integrate the action of PacC and ZafA into gene specific transcriptional responses.

Automated summary

This study investigated the regulation of gene expression in Aspergillus fumigatus under different environmental conditions. It was found that the PacC transcription factor plays a crucial role in modulating gene expression under acidic and alkaline zinc-limiting conditions, and proposed the involvement of a third factor in integrating the actions of PacC and ZafA for gene-specific transcriptional responses.