期刊
EMBO REPORTS
卷 22, 期 4, 页码 -出版社
WILEY
DOI: 10.15252/embr.202051030
关键词
AURKA; centrosome; meiotic spindle; PCM; PLK1
资金
- Ministerio de Economia, Industria y Competitividad, Gobierno de Espana (MINECO) (Spain) [BFU2014-53681-P, BFU2015-71786-REDT, RTI2018-095582-B-I00]
- grant Ayuda para Estudios de Master from the Department of Biology of the Universidad Autonoma de Madrid (Spain)
- grant Ayudas PostMaster from the Department of Biology of the Universidad Autonoma de Madrid (Spain)
- Ministry of Education of Spain (FPI grants)
The study reveals that during meiotic divisions in male mice, centrosomes duplicate twice to form bipolar spindles, with Polo-like kinase 1 playing a central role in centrosome assembly, maturation, migration, and spindle formation during spermatogenesis. Inhibition of both Polo-like kinase 1 and Aurora A in organotypic cultures of seminiferous tubules highlights their prominent role in regulating the formation of meiotic bipolar spindles.
Cell division requires the regulation of karyokinesis and cytokinesis, which includes an essential role of the achromatic spindle. Although the functions of centrosomes are well characterised in somatic cells, their role during vertebrate spermatogenesis remains elusive. We have studied the dynamics of the meiotic centrosomes in male mouse during both meiotic divisions. Results show that meiotic centrosomes duplicate twice: first duplication occurs in the leptotene/zygotene transition, while the second occurs in interkinesis. The maturation of duplicated centrosomes during the early stages of prophase I and II are followed by their separation and migration to opposite poles to form bipolar spindles I and II. The study of the genetic mouse model Plk1(Delta/Delta) indicates a central role of Polo-like kinase 1 in pericentriolar matrix assembly, in centrosome maturation and migration, and in the formation of the bipolar spindles during spermatogenesis. In addition, in vitro inhibition of Polo-like kinase 1 and Aurora A in organotypic cultures of seminiferous tubules points out to a prominent role of both kinases in the regulation of the formation of meiotic bipolar spindles.
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