期刊
ELECTROPHORESIS
卷 42, 期 3, 页码 245-256出版社
WILEY
DOI: 10.1002/elps.202000223
关键词
Capillary‐ channeled polymer (C‐ CP) fibers; Extracellular vesicles; Hydrophobic interaction chromatography (HIC); Mass spectrometry; Proteomics; Serum
资金
- NCI NIH HHS [R01 CA160254, R21 CA189775, R50 CA221808] Funding Source: Medline
- NIGMS NIH HHS [R01 GM049500] Funding Source: Medline
A rapid, low-cost, and simple separation strategy has been developed to isolate extracellular vesicles from small serum samples with minimal contamination, achieving high purity for EV proteome analysis. The method utilizes a PET C-CP fiber phase/HIC approach for EV separation and a post-column cleanup protocol to eliminate non-EV proteins, resulting in successful isolation of exosome-like particles with high yield in approximately 15 minutes. This methodology addresses major challenges in EV separation for proteomics analysis and has the potential to be combined with other techniques for further purification.
We have developed a rapid, low-cost, and simple separation strategy to separate extracellular vesicles (EVs) from a small amount of serum (i.e.,<100 mu L) with minimal contamination by serum proteins and lipoprotein particles to meet the high purity requirement for EV proteome analysis. EVs were separated by a novel polyester capillary channel polymer (PET C-CP) fiber phase/hydrophobic interaction chromatography (HIC) method which is rapid and can process small size samples. The collected EV fractions were subjected to a post-column cleanup protocol using a centrifugal filter to perform buffer exchange and eliminate potential coeluting non-EV proteins while minimizing EV sample loss. Downstream characterization demonstrated that our current strategy can separate EVs with the anticipated exosome-like particle size distribution and high yield (similar to 1 x 10(11) EV particles per mL of serum) in approximately 15 min. Proteome profiling of the EVs reveals that a group of genuine EV components were identified that have significantly less high-abundance blood proteins and lipoprotein particle contamination in comparison to traditional separation methods. The use of this methodology appears to address the major challenges facing EV separation for proteomics analysis. In addition, the EV post-column cleanup protocol proposed in the current work has the potential to be combined with other separation methods, such as ultracentrifugation (UC), to further purify the separated EV samples.
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